Association of Tissue Transglutaminase with Nerve Growth Factor, Prolidase Activity and Oxidative Stress in Celiac Patients
Published: November 1, 2018 | DOI: https://doi.org/10.7860/JCDR/2018/37282.12197
Akhilesh Kumar Verma, Ankur Sharma, BK Das, Shalabh Srivastava, Ragini Srivastava
1. Senior Research Fellow, Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
2. Junior Resident, Department of Paediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
3. Professor, Department of Paediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
4. Associate Professor, Department of Oral Pathology, Jaipur Dental College, Jaipur, Rajasthan, India.
5. Associate Professor, Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Correspondence
Dr. Ragini Srivastava,
Associate Professor, Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University,
Varanasi-221005, Uttar Pradesh, India.
E-mail: ragsriva@gmail.com
Introduction: Celiac Disease (CD) is an autoimmune gastrointestinal disorder; it is characterised by chronic inflammation and elevation of tissue Transglutaminase Immunoglobulin-A (tTG-IgA). Nerve Growth Factor (NGF) and prolidase enzyme are elevated in different autoimmune and chronic inflammatory diseases; however, the status of NGF and prolidase enzyme remains unexplored in celiac patients till date.
Aim: To evaluate the correlation of tTG-IgA to NGF, Serum Prolidase Activity (SPA) and oxidative stress in the CD patients.
Materials and Methods: The study was conducted on a total of 92 subjects, 46 patients with CD and remaining 46 healthy controls. The tTG-IgA and NGF were measured with the use of ELISA kit. SPA and oxidative stress were measured spectrophotometrically.
Results: The tTG-IgA, NGF, SPA, Total Oxidant Status (TOS) and Oxidative Stress Index (OSI) were significantly elevated in the serum of patients as compared to controls (all p<0.001); however, Total Antioxidant Status (TAS) was significantly decreased (p<0.001). The tTG-IgA showed a positive correlation with NGF, SPA, TOS and OSI in the patients (all p<0.001); while it was negatively correlated to TAS (p<0.001).
Conclusion: The present study concluded that elevated NGF, prolidase enzyme, tTG and oxidative stress might be associated with the pathogenesis of celiac disease.
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